Proteintech, 84643-1-RR, IGF2 Recombinant monoclonal antibody

Size: 20ul / 100ul The IGF2 (84643-1-RR) by Proteintech is a Recombinant antibody targeting IGF2 in WB, IHC, ELISA applications with reactivity to human samples 84643-1-RR targets IGF2 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HuH-7 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Insulin-like growth factor 2 (IGF2) is a crucial protein that plays a significant role in regulating growth, particularly during normal fetal development, and is also often dysregulated during tumorigenesis. IGF2 is one of the main ligands of the type 1 IGF receptor (IGF-1R), a tyrosine kinase receptor that transmits signals upon binding with IGF-II. IGF2 is predominantly secreted via the placenta during pregnancy and, after birth, primarily by hepatocytes, with its secretion being independent of growth hormone (GH) secretion, unlike IGF-I. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: insulin-like growth factor 2 (somatomedin A) Observed Molecular Weight: 20 kDa Gene Symbol: IGF2 Gene ID (NCBI): 3481 RRID: AB_3672102 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P01344 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.