Proteintech, 84706-4-RR, IBTK Recombinant monoclonal antibody

Size: 20ul / 100ul The IBTK (84706-4-RR) by Proteintech is a Recombinant antibody targeting IBTK in WB, IHC, ELISA applications with reactivity to human samples 84706-4-RR targets IBTK in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HepG2 cells, Jurkat cells, Ramos cells Positive IHC detected in: mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information IBTK, also known as BTKI and KIAA1417, which is involved in stress response and tumor growth. It has 3 isoforms and has been found to be hyper-expressed in chronic lymphocytic leukemia and correlated with disease progression (PMID: 29317636). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag35421 Product name: Recombinant human IBTK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 850-1000 aa of BC143745 Sequence: VADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAKTKAKKKPRKRSDSSGGYNLSD Predict reactive species Full Name: inhibitor of Bruton agammaglobulinemia tyrosine kinase Observed Molecular Weight: 160 kDa GenBank Accession Number: BC143745 Gene Symbol: IBTK Gene ID (NCBI): 25998 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9P2D0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.