Proteintech, 84719-4-RR, MPP8 Recombinant monoclonal antibody

Size: 20ul / 100ul The MPP8 (84719-4-RR) by Proteintech is a Recombinant antibody targeting MPP8 in WB, ELISA applications with reactivity to human samples 84719-4-RR targets MPP8 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10268 Product name: Recombinant human MPHOSPH8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC003542 Sequence: MEQVAEGARVTAVPVSAADSTEELAEVEEGVGVVGEDNDAAARGAEAFGDSEEDGEDVFEVEKILDMKTEGGKVLYKVRWKGYTSDDDTWEPEIHLEDCKEVLLEFRKKIAENKAKAVRKDIQRLSLNNDIFEANSDSDQQSETKEDTSPKKKKKKLRQREEKSPDDLKKKKAKAGKLKDKSKPDLESSLESLVFDLRTKKRISEAKEELKESKKPKKDEVKETKELKKVKKGEIRDLKTKTREDPKENRKTKKEKFVESQVESESSVLNDSPFPEDDSEGLHSDSREEKQNTKSARERAG Predict reactive species Full Name: M-phase phosphoprotein 8 Calculated Molecular Weight: 860 aa, 97 kDa Observed Molecular Weight: 97-105 kDa GenBank Accession Number: BC003542 Gene Symbol: MPP8 Gene ID (NCBI): 54737 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q99549 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.