Proteintech, 84769-4-RR, HMGCL Recombinant monoclonal antibody

Size: 20ul / 100ul The HMGCL (84769-4-RR) by Proteintech is a Recombinant antibody targeting HMGCL in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 84769-4-RR targets HMGCL in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, rat liver tissue, mouse kidney tissue, rat kidney tissue Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10426 Product name: Recombinant human HMGCL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC010570 Sequence: MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL Predict reactive species Full Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase Calculated Molecular Weight: 325 aa, 34 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC010570 Gene Symbol: HMGCL Gene ID (NCBI): 3155 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P35914 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.