Proteintech, 84774-7-RR, ACSS2 Recombinant monoclonal antibody

Size: 20ul / 100ul The ACSS2 (84774-7-RR) by Proteintech is a Recombinant antibody targeting ACSS2 in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 84774-7-RR targets ACSS2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, Caco-2 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue Positive FC (Intra) detected in: HepG2 cells, Caco-2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9121 Product name: Recombinant human ACSS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 185-534 aa of BC010141 Sequence: VFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDVNRLWSIVDKYKVTKFYTAPTAIRLLMKFGDEPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGAQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHERFETTYFKKFPGYYVTGDGCQRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPKLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDMSTVADPSVISHLFSHRCLTIQ Predict reactive species Full Name: acyl-CoA synthetase short-chain family member 2 Calculated Molecular Weight: 701 aa, 79 kDa Observed Molecular Weight: 79 kDa GenBank Accession Number: BC010141 Gene Symbol: ACSS2 Gene ID (NCBI): 55902 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9NR19 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.