Proteintech, 84783-2-RR, FGD1 Recombinant monoclonal antibody

Size: 20ul / 100ul The FGD1 (84783-2-RR) by Proteintech is a Recombinant antibody targeting FGD1 in WB, ELISA applications with reactivity to human samples 84783-2-RR targets FGD1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SK-N-SH cells, U2OS cells, U-87 MG cells, BxPC-3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3861 Product name: Recombinant human FGD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 611-961 aa of BC034530 Sequence: YLILFNDRLLYCVPRLRLLGQKFSVRARIDVDGMELKESSNLNLPRTFLVSGKQRSLELQARTEEEKKDWVQAINSTLLKHEQTLETFKLLNSTNREDEDTPPNSPNVDLGKRAPTPIREKEVTMCMRCQEPFNSITKRRHHCKACGHVVCGKCSEFRARLVYDNNRSNRVCTDCYVALHGVPGSSPACSQHTPQRRRSILEKQASVAAENSVICSFLHYMEKGGKGWHKAWFVVPENEPLVLYIYGAPQDVKAQRSLPLIGFEVGPPEAGERPDRRHVFKITQSHLSWYFSPETEELQRRWMAVLGRAGRGDTFCPGPTLSEDREMEEAPVAALGATAEPPESPQTRDKT Predict reactive species Full Name: FYVE, RhoGEF and PH domain containing 1 Calculated Molecular Weight: 901 aa, 91 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: BC034530 Gene Symbol: FGD1 Gene ID (NCBI): 2245 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P98174 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.