Proteintech, 84790-2-RR, Siglec-E Recombinant monoclonal antibody

Size: 20ul / 100ul The Siglec-E (84790-2-RR) by Proteintech is a Recombinant antibody targeting Siglec-E in WB, IHC, ELISA applications with reactivity to mouse samples 84790-2-RR targets Siglec-E in WB, IHC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse spleen tissue Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: PMID: 9498912; 22749793). Mouse Siglec‑E, also known as myeloid inhibitory siglec (MIS), is a mouse CD33-related siglec that contains three extracellular immunoglobulin-like domains, a transmembrane region and a cytoplasmic tail bearing two immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which can recruit SHP-1 and SHP-2, two inhibitory regulators of immunoreceptor signal transduction (PMID: 11171044). Siglec-E is an inhibitory receptor and is expressed broadly on cells of innate immune cells including neutrophils, monocytes, macrophages, dendritic cells, and a subset of natural killer cells (PMID: 15048729; 31546700). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg2733 Product name: Recombinant Mouse Siglec-12 protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 19-350 aa of NM_031181.2 Sequence: QNPQEVFTLNVERKVVVQEGLCVLVPCNFSYLKKRLTDWTDSDPVHGFWYREGTDRRKDSIVATNNPIRKAVKETRNRFFLLGDPWRNDCSLNIREIRKKDAGLYFFRLERGKTKYNYMWDKMTLVVTALTNTPQILLPETLEAGHPSNLTCSVPWDCGWTAPPIFSWTGTSVSFLSTNTTGSSVLTITPQPQDHGTNLTCQVTLPGTNVSTRMTIRLNVSYAPKNLTVTIYQGADSVSTILKNGSSLPISEGQSLRLICSTDSYPPANLSWSWDNLTLCPSKLSKPGLLELFPVHLKHGGVYTCQAQHALGSQHISLSLSPQSSATLSEMM Predict reactive species Full Name: sialic acid binding Ig-like lectin E Calculated Molecular Weight: 52 kDa Observed Molecular Weight: 70-90 kDa GenBank Accession Number: NM_031181.2 Gene Symbol: Siglec-E Gene ID (NCBI): 83382 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q91Y57 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.