Proteintech, 84792-5-RR, MCM3 Recombinant monoclonal antibody

Size: 20ul / 100ul The MCM3 (84792-5-RR) by Proteintech is a Recombinant antibody targeting MCM3 in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 84792-5-RR targets MCM3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, K-562 cells, Raji cells, NIH/3T3 cells, C6 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HeLa cells, K-562 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7966 Product name: Recombinant human MCM3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 201-503 aa of BC001626 Sequence: IQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLR Predict reactive species Full Name: minichromosome maintenance complex component 3 Calculated Molecular Weight: 91 kDa Observed Molecular Weight: 102-105 kDa GenBank Accession Number: BC001626 Gene Symbol: MCM3 Gene ID (NCBI): 4172 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P25205 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.