Proteintech, 84800-1-RR, GPD1 Recombinant monoclonal antibody

Size: 20ul / 100ul The GPD1 (84800-1-RR) by Proteintech is a Recombinant antibody targeting GPD1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 84800-1-RR targets GPD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, mouse liver tissue Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information GPD1 (Glycerol-3-phosphate dehydrogenase 1) is an important enzyme belonging to the NAD-dependent glycerol-3-phosphate dehydrogenase family. Its C-terminal structural domain contains multiple helical structures for binding the substrate DHAP, and its N-terminal structural domain contains a β-folded core for binding NADH. GPD1 catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotinamide adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+, and plays a key role in carbohydrate and lipid metabolism. GPD1 also works with mitochondrial glycerol-3-phosphate dehydrogenase to form a glycerophosphate shuttle system that facilitates the transfer of reducing equivalents from the cytoplasm to the mitochondria. Abnormal activity of GPD1 has been associated with a variety of metabolic disorders, such as obesity, hypertriglyceridemia, and GPD1 has been implicated in cancer, potentially acting as a tumor suppressor. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag4278 Product name: Recombinant human GPD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC032234 Sequence: MASKKVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM Predict reactive species Full Name: glycerol-3-phosphate dehydrogenase 1 (soluble) Calculated Molecular Weight: 349 aa, 38 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC032234 Gene Symbol: GPD1 Gene ID (NCBI): 2819 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P21695 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.