Proteintech, 84814-1-RR, 6*His, His-Tag Recombinant monoclonal antibody

Size: 100ul The 6*His, His-Tag (84814-1-RR) by Proteintech is a Recombinant antibody targeting 6*His, His-Tag in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to recombinant protein samples 84814-1-RR targets 6*His, His-Tag in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with recombinant protein samples. Tested Applications Positive WB detected in: Transfected HEK-293T cells Positive IF/ICC detected in: HEK-293 cells Positive FC (Intra) detected in: Transfected HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein. Specification Tested Reactivity: recombinant protein Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: 6*His, His-Tag Calculated Molecular Weight: 1 kDa RRID: AB_3672138 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: HISTAG Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.