Proteintech, 84845-3-RR, HCN1 Recombinant monoclonal antibody

Size: 20ul / 100ul The HCN1 (84845-3-RR) by Proteintech is a Recombinant antibody targeting HCN1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 84845-3-RR targets HCN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information HCN1, also named as BCNG1, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. HCN1 contributes to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. HCN1 may mediate responses to sour stimuli. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 1 Calculated Molecular Weight: 99 kDa Observed Molecular Weight: 99-120 kDa GenBank Accession Number: NM_021072 Gene Symbol: HCN1 Gene ID (NCBI): 348980 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O60741 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.