Proteintech, 84873-4-RR, HSPA1B Recombinant monoclonal antibody

Size: 20ul / 100ul The HSPA1B (84873-4-RR) by Proteintech is a Recombinant antibody targeting HSPA1B in WB, ELISA applications with reactivity to human, mouse, rat samples 84873-4-RR targets HSPA1B in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, A431 cells, mouse brain tissue, rat brain tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSP70 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSP70 is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSP70 protein can be highly activated by various stressful stimuli. Significant up-regulation of HSP70 has been found in various tumors. Recently it has been reported that HSP70 can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag13700 Product name: Recombinant human HSPA1B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 292-641 aa of BC057397 Sequence: DFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD Predict reactive species Full Name: heat shock 70kDa protein 1B Calculated Molecular Weight: 70 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC057397 Gene Symbol: HSP70 Gene ID (NCBI): 3304 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P08107 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.