Proteintech, 84881-2-RR, Choline Kinase Alpha Recombinant monoclonal antibody

Size: 20ul / 100ul The Choline Kinase Alpha (84881-2-RR) by Proteintech is a Recombinant antibody targeting Choline Kinase Alpha in WB, ELISA applications with reactivity to human samples 84881-2-RR targets Choline Kinase Alpha in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, COLO 320 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag4761 Product name: Recombinant human CHKA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 139-439 aa of BC036471 Sequence: EPRKVLLRLYGAILQVGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELGLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGQWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV Predict reactive species Full Name: choline kinase alpha Calculated Molecular Weight: 439 aa, 52 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC036471 Gene Symbol: CHKA Gene ID (NCBI): 1119 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35790 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.