Proteintech, 84900-1-RR, RPL15 Recombinant monoclonal antibody

Size: 20ul / 100ul The RPL15 (84900-1-RR) by Proteintech is a Recombinant antibody targeting RPL15 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 84900-1-RR targets RPL15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, COLO 320 cells, NCI-H1299 cells, mouse colon tissue, rat colon tissue Positive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information RPL15 (ribosomal protein L15) is a component of the ribosomal large subunit 60S, participating in the assembly process of ribosomal subunits and involved in the processing of rRNA. Moreover, RPL15 deletion will lead to abnormalities in the biogenesis of ribosomal subunits. The abnormal expression or mutation of RPL15 protein is also closely related to the occurrence and development of many diseases. RPL15 was highly expressed in gastric cancer cell lines, and interference with the expression of RPL15 could inhibit gastric cancer cell growth. In addition, RPL15 in skin squamous cell carcinoma tissues and pancreatic cancer cell lines decreased sharply, which had a great relationship with the survival rate. PMID:35410346 Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9948 Product name: Recombinant human RPL15 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-204 aa of BC070328 Sequence: MGAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGGRKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR Predict reactive species Full Name: ribosomal protein L15 Calculated Molecular Weight: 204 aa, 24 kDa Observed Molecular Weight: 27 kDa GenBank Accession Number: BC070328 Gene Symbol: RPL15 Gene ID (NCBI): 6138 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P61313 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.