Proteintech, 84906-1-RR, Phospho-EGFR (Tyr1197) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-EGFR (Tyr1197) (84906-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-EGFR (Tyr1197) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 84906-1-RR targets Phospho-EGFR (Tyr1197) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: EGF treated A431 cells Positive IF/ICC detected in: EGF treated A431 cells Positive FC (Intra) detected in: EGF treated A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). The EGFR autophosphorylation sites Tyr-992 and Tyr-1173 (also known as Tyr 1197) have been shown to play a critical role in the activation of the MAPK cascade following EGF stimulation. (PMID: 15708852) Some scientists call EGFR Tyr1197 as EGFR Tyr1173, the site where the signaling peptide is removed. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Calculated Molecular Weight: 1210 aa, 134 kDa Observed Molecular Weight: 175 kDa GenBank Accession Number: BC094761 Gene Symbol: EGFR Gene ID (NCBI): 1956 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P00533 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.