Proteintech, 84914-4-RR, TCOF1 Recombinant monoclonal antibody

Size: 20ul / 100ul The TCOF1 (84914-4-RR) by Proteintech is a Recombinant antibody targeting TCOF1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 84914-4-RR targets TCOF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, A549 cells, HEK-293T cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1447 Product name: Recombinant human TCOF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC014559 Sequence: MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSE Predict reactive species Full Name: Treacher Collins-Franceschetti syndrome 1 Calculated Molecular Weight: 97 kDa, 144 kDa, 152 kDa Observed Molecular Weight: 220-240 kDa GenBank Accession Number: BC014559 Gene Symbol: TCOF1 Gene ID (NCBI): 6949 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13428 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.