Proteintech, 84944-4-RR, BID Recombinant monoclonal antibody

Size: 20ul / 100ul The BID (84944-4-RR) by Proteintech is a Recombinant antibody targeting BID in WB, FC (Intra), ELISA applications with reactivity to human samples 84944-4-RR targets BID in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, A431 cells, A549 cells, HEK-293 cells, L-929 cells Positive FC (Intra) detected in: HeLa cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: BH3 interacting domain death agonist Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 22 kDa GenBank Accession Number: BC009197 Gene Symbol: BID Gene ID (NCBI): 637 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P55957 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.