Proteintech, 84973-1-RR, LRP4 Recombinant monoclonal antibody

Size: 20ul / 100ul The LRP4 (84973-1-RR) by Proteintech is a Recombinant antibody targeting LRP4 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 84973-1-RR targets LRP4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: rat brain tissue, mouse brain tissue, PC-3 cells Positive IF/ICC detected in: PC-12 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information LRP4 (low-density lipoprotein receptor-related protein 4), also known as multiple epidermal growth factor-like domains 7 (MEGF7), is a type I single transmembrane protein of the low-density lipoprotein receptor (LDLR) family. LRP4 is composed of a large extracellular region containing multiple LDLa repeats, EGF-like domains and β-propeller domains, a transmembrane region and a cytoplasmic region containing an NPxY motif and a PDZ-interacting motif (PMID: 26071838). LRP4 binds agrin and recruits it to the MuSK signaling complex, which is critical for neuromuscular junction formation (PMID: 17119023; 18957220). LPR4 mutation or malfunction is implicated in disorders including congenital myasthenic syndrome, myasthenia gravis, and diseases of bone or kidney (26071838). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag19413 Product name: Recombinant human LRP4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1561-1905 aa of BC136667 Sequence: ALTQQDRWIYWTDWQTKSIQRVDKYSGRNKETVLANVEGLMDIIVVSPQRQTGTNACGVNNGGCTHLCFARASDFVCACPDEPDSQPCSLVPGLVPPAPRATGMSEKSPVLPNTPPTTLYSSTTRTRTSLEEVEGRCSERDARLGLCARSNDAVPAAPGEGLHISYAIGGLLSILLILVVIAALMLYRHKKSKFTDPGMGNLTYSNPSYRTSTQEVKIEAIPKPAMYNQLCYKKEGGPDHNYTKEKIKIVEGICLLSGDDAEWDDLKQLRSSRGGLLRDHVCMKTDTVSIQASSGSLDDTETEQLLQEEQSECSSVHTAATPERRGSLPDTGWKHERKLSSESQV Predict reactive species Full Name: low density lipoprotein receptor-related protein 4 Calculated Molecular Weight: 1905 aa, 212 kDa Observed Molecular Weight: 245 kDa GenBank Accession Number: BC136667 Gene Symbol: LRP4 Gene ID (NCBI): 4038 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O75096 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.