Proteintech, 84999-4-RR, ATP1A1/2/3/4 Recombinant monoclonal antibody

Size: 20ul / 100ul The ATP1A1/2/3/4 (84999-4-RR) by Proteintech is a Recombinant antibody targeting ATP1A1/2/3/4 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 84999-4-RR targets ATP1A1/2/3/4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: 37°C incubated mouse brain tissue, 37°C incubated rat brain tissue Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4. The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognizes all the isoforms of α subunit. For optimal WB detection with ATP1A2, we do not recommend boiling the sample after lysis. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10489 Product name: Recombinant human ATP1A2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 347-700 aa of BC052271 Sequence: KRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGATFDKRSPTWTALSRIAGLCNRAVFKAGQENISVSKRDTAGDASESALLKCIELSCGSVRKMRDRNPKVAEIPFNSTNKYQLSIHEREDSPQSHVLVMKGAPERILDRCSTILVQGKEIPLDKEMQDAFQNAYMELGGLGERVLGFCQLNLPSGKFPRGFKFDTDELNFPTEKLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPMSQVNPREAKACVVHGSDLKDMTSEQLDEILKNHTEIVFARTSPQQKLIIVE Predict reactive species Full Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide Calculated Molecular Weight: 1020 aa, 112 kDa Observed Molecular Weight: 97-100 kDa GenBank Accession Number: BC052271 Gene Symbol: ATP1A2 Gene ID (NCBI): 477 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P50993 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.