Proteintech, 85032-2-RR, RFK Recombinant monoclonal antibody

Size: 20ul / 100ul The RFK (85032-2-RR) by Proteintech is a Recombinant antibody targeting RFK in WB, ELISA applications with reactivity to human samples 85032-2-RR targets RFK in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, HEK-293 cells, U-87 MG cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag8591 Product name: Recombinant human RFK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC007069 Sequence: MPRADCIMRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH Predict reactive species Full Name: riboflavin kinase Calculated Molecular Weight: 162 aa, 18 kDa Observed Molecular Weight: 15~18 kDa GenBank Accession Number: BC007069 Gene Symbol: RFK Gene ID (NCBI): 55312 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q969G6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.