Proteintech, 85066-5-RR, CD27 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD27 (85066-5-RR) by Proteintech is a Recombinant antibody targeting CD27 in WB, IF/ICC, IF-P, ELISA applications with reactivity to human samples 85066-5-RR targets CD27 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Ramos cells Positive IF-P detected in: human tonsillitis tissue Positive IF/ICC detected in: Ramos cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)-P: IF-P : 1:300-1:1200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: CD27 molecule Calculated Molecular Weight: 29 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC012160 Gene Symbol: CD27 Gene ID (NCBI): 939 ENSEMBL Gene ID: ENSG00000139193 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P26842 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.