Proteintech, 85196-3-RR, Caspase 10 Recombinant monoclonal antibody

Size: 20ul / 100ul The Caspase 10 (85196-3-RR) by Proteintech is a Recombinant antibody targeting Caspase 10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 85196-3-RR targets Caspase 10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, Jurkat cells, A431 cells, K-562 cells Positive IHC detected in: Jurkat cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Jurkat cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). The full length has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: caspase 10, apoptosis-related cysteine peptidase Calculated Molecular Weight: 59 kDa Observed Molecular Weight: 59 kDa GenBank Accession Number: BC042844 Gene Symbol: Caspase 10 Gene ID (NCBI): 843 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q92851 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.