Proteintech, 85202-1-RR, Filamin B Recombinant monoclonal antibody

Size: 20ul / 100ul The Filamin B (85202-1-RR) by Proteintech is a Recombinant antibody targeting Filamin B in IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 85202-1-RR targets Filamin B in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: human hepatocellular cancer, human colon tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, A549 cells Positive FC (Intra) detected in: HEK-293T cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: filamin B, beta (actin binding protein 278) Calculated Molecular Weight: 278 kDa GenBank Accession Number: NM_001457 Gene Symbol: FLNB Gene ID (NCBI): 2317 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O75369 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.