Proteintech, 85205-1-RR, CCKAR-specific Recombinant monoclonal antibody

Size: 20ul / 100ul The CCKAR-specific (85205-1-RR) by Proteintech is a Recombinant antibody targeting CCKAR-specific in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 85205-1-RR targets CCKAR-specific in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SGC-7901 cells, mouse stomach tissue, rat stomach tissue, mouse pancreas tissue, rat pancreas tissue, mouse bladder tissue, mouse lung tissue Positive IP detected in: mouse lung tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Cholecystokinin (CCK) receptor, including CCKAR and CCKBR, is a receptor for cholecystokinin, mainly regulating pancreatic growth and enzyme secretion, and smooth muscle contraction of the gallbladder and stomach (PMID: 34556863). CCKAR (Cholecystokinin receptor type A) is primarily expressed in the alimentary tract, while CCKBR is mainly found in the brain and the stomach (PMID: 16816139). CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer (PMID: 36733361). The calculated MW of CCKAR is 48 kDa, but the observed MW is around 75 kDa, which may be with glycosylation modification. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cholecystokinin A receptor Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: NM_000730 Gene Symbol: CCKAR Gene ID (NCBI): 886 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P32238 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.