Proteintech, 85220-5-RR, DFFA,DFF45 Recombinant monoclonal antibody

Size: 20ul / 100ul The DFFA,DFF45 (85220-5-RR) by Proteintech is a Recombinant antibody targeting DFFA,DFF45 in WB, IF/ICC, ELISA applications with reactivity to human, rat samples 85220-5-RR targets DFFA,DFF45 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, HEK-293 cells, K-562 cells, MCF-7 cells, C6 cells, rat brain tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0244 Product name: Recombinant human DFFA,DFF45 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 9-328 aa of BC000037 Sequence: GVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQ Predict reactive species Full Name: DNA fragmentation factor, 45kDa, alpha polypeptide Calculated Molecular Weight: 37 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC000037 Gene Symbol: DFFA Gene ID (NCBI): 1676 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O00273 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.