Proteintech, 85238-1-RR, Phospho-IRS1 (Ser307) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-IRS1 (Ser307) (85238-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-IRS1 (Ser307) in WB, ELISA applications with reactivity to human, mouse samples 85238-1-RR targets Phospho-IRS1 (Ser307) in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, TNF alpha treated HeLa cells, TNF alpha treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Serine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been difficult to determine. It has been confirmed that JNK and IKK are involved in the phosphorylation of IRS-1 (Ser307/312). Additionally, activation of Akt, ERK, mTOR, and PKCζ correlates to IRS-1 phosphorylation at Ser307/312, Ser267/270, and Ser612/616 in rodent/human IRS-1. (PMID: 11606564, PMID: 12714600) Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: insulin receptor substrate 1 Calculated Molecular Weight: 1242 aa, 132 kDa Observed Molecular Weight: 180 kDa GenBank Accession Number: BC053895 Gene Symbol: IRS1 Gene ID (NCBI): 3667 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35568 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.