Proteintech, 85245-6-RR, WWTR1/TAZ Recombinant monoclonal antibody

Size: 20ul / 100ul The WWTR1/TAZ (85245-6-RR) by Proteintech is a Recombinant antibody targeting WWTR1/TAZ in WB, ELISA applications with reactivity to human samples 85245-6-RR targets WWTR1/TAZ in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, A549 cells, LN-229 cells, MDA-MB-453 cells, NIH/3T3 cells, HSC-T6 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma (PMID: 22003437, PMID: 27764783). TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg6470 Product name: Recombinant Human WWTR1 protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 1-400 aa of NM_001168278.2 Sequence: MNPASAPPPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSSTPVPQRSMAVSQPNLVMNHQHQQQMAPSTLSQQNHPTQNPPAGLMSMPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMEAETLAPVQAAVNPPTMTPDMRSITNNSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNVDEMDTGENAGQTPMNINPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL Predict reactive species Full Name: WW domain containing transcription regulator 1 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: NM_001168278.2 Gene Symbol: WWTR1 Gene ID (NCBI): 25937 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9GZV5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.