Proteintech, 85325-2-RR, RUNX1/AML1 Recombinant monoclonal antibody

Size: 20ul / 100ul The RUNX1/AML1 (85325-2-RR) by Proteintech is a Recombinant antibody targeting RUNX1/AML1 in WB, ELISA applications with reactivity to human, mouse, rat samples 85325-2-RR targets RUNX1/AML1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PMA treated THP-1 cells, THP-1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in the diversification of sensory neurons. RUNX1 exists in some isoforms with a range of MW 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa; however, the modified protein ranges from approximately 49 to 55 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: runt-related transcription factor 1 Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: NM_001754 Gene Symbol: RUNX1 Gene ID (NCBI): 861 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q01196 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.