Proteintech, 85364-3-RR, TRIM33 Recombinant monoclonal antibody

Size: 20ul / 100ul The TRIM33 (85364-3-RR) by Proteintech is a Recombinant antibody targeting TRIM33 in WB, IF/ICC, ELISA applications with reactivity to human samples 85364-3-RR targets TRIM33 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, COLO 320 cells, MOLT-4 cells Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TRIM33, also named KIAA1113, RFG7, and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion, and degradation via the ubiquitin-proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight detected is about 140-150 kDa the same as the result from the paper(PMID:25639486;26592194). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tripartite motif-containing 33 Calculated Molecular Weight: 122 kDa Observed Molecular Weight: 140-150 kDa GenBank Accession Number: NM_015906 Gene Symbol: TRIM33 Gene ID (NCBI): 51592 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9UPN9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.