Proteintech, 85397-4-RR, DYNC1H1 Recombinant monoclonal antibody

Size: 20ul / 100ul The DYNC1H1 (85397-4-RR) by Proteintech is a Recombinant antibody targeting DYNC1H1 in WB, ELISA applications with reactivity to human, mouse samples 85397-4-RR targets DYNC1H1 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, HEK-293T cells, NIH/3T3 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Dyneins are a group of microtubule-activated ATPases that convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein comprises 2 heavy chain polypeptides and several intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2999 Product name: Recombinant human KIAA0325 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 590-989 aa of BC021297 Sequence: MAQSIYGGRVDNEFDQRLLNTFLERLFTTRSFDSEFKLACKVDGHKDIQMPDGIRREEFVQWVELLPDTQTPSWLGLPNNAERVLLTTQGVDMISKMLKMQMLEDEDDLAYAETEKKTRTDSTSDGRPAWMRTLHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTLINELVKGILPRSWSHYTVPAGMTVIQWVSDFSERIKQLQNISLAAASGGAKELKNIHVCLGGLFVPEAYITATRQYVAQANSWSLEELCLEVNVTTSQGATLDACSFGVTGLKLQGATCNNNKLSLSNAISTALPLTQLRWVKQTNTEKKASVVTLPVYLNFTRADLIFTVDFEIATKEDPRSFYERGVAVLCTE Predict reactive species Full Name: dynein, cytoplasmic 1, heavy chain 1 Calculated Molecular Weight: 4646 aa, 532 kDa Observed Molecular Weight: 532 kDa GenBank Accession Number: BC021297 Gene Symbol: DYNC1H1 Gene ID (NCBI): 1778 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q14204 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.