Proteintech, 85419-4-RR, PTGES2 Recombinant monoclonal antibody

Size: 20ul / 100ul The PTGES2 (85419-4-RR) by Proteintech is a Recombinant antibody targeting PTGES2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 85419-4-RR targets PTGES2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HeLa cells, mouse heart tissue, HepG2 cells, HEK-293 cells, Jurkat cells, rat heart tissue, pig heart tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562). Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1329 Product name: Recombinant human PTGES2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC011613 Sequence: MDPAARVVRALWPGGCALAWRLGGRPQPLLPTQSRAGFAGAAGGPSPVAAARKGSPRLLGAAALALGGALGLYHTARWHLRAQDLHAERSAAQLSLSSRLQLTLYQYKTCPFCSKVRAFLDFHALPYQVVEVNPVRRAEIKFSSYRKVPILVAQEGESSQQLNDSSVIISALKTYLVSGQPLEEIITYYPAMKAVNEQGKEVTEFGNKYWLMLNEKEAQQVYGGKEARTEEMKWRQWADDWLVHLISPNVYRTPTEALASFDYIVREGKFGAVEGAVAKYMGAAAMYLISKRLKSRHRLQDNVREDLYEAADKWVAAVGKDRPFMGGQKPNLADLAVYGVLRVMEGLDAFDDLMQHTHIQPWYLRVERAITEASPAH Predict reactive species Full Name: prostaglandin E synthase 2 Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 30-35 kDa GenBank Accession Number: BC011613 Gene Symbol: PTGES2 Gene ID (NCBI): 80142 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9H7Z7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.