Proteintech, 85423-2-RR, Cytokeratin 6B Recombinant monoclonal antibody

Size: 20ul / 100ul The Cytokeratin 6B (85423-2-RR) by Proteintech is a Recombinant antibody targeting Cytokeratin 6B in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 85423-2-RR targets Cytokeratin 6B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, HaCaT cells, mouse skin tissue, rat skin tissue, human saliva Positive IHC detected in: human cervical cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Cytokeratin 6B (CK6B) is a type of intermediate filament protein primarily expressed in certain epithelial cells. Cytokeratin 6B consists of a central α-helical rod domain flanked by non-helical head and tail domains, which is a common structural feature of cytokeratins. CK6B is mainly expressed in the basal and suprabasal layers of stratified epithelia, such as the oral mucosa, esophagus, and conjunctiva. It is also found in some specialized epithelial cells like the hair follicle outer root sheath and the nail matrix. CK6B can serve as a diagnostic marker for certain pathological conditions. Its expression pattern can be used to distinguish between different types of epithelial tumors or to identify specific epithelial cell populations in histopathological studies. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag11288 Product name: Recombinant human KRT6B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 214-564 aa of BC034535 Sequence: FEQYINNLRRQLDNIVGERGRLDSELRNMQDLVEDLKNKYEDEINKRTAAENEFVTLKKDVDAAYMNKVELQAKADTLTDEINFLRALYDAELSQMQTHISDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIAQRSRAEAESWYQTKYEELQITAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISVVQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRATGGGLSSVGGGSSTIKYTTTSSSSRKSYKH Predict reactive species Full Name: keratin 6B Calculated Molecular Weight: 564 aa, 60 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC034535 Gene Symbol: Cytokeratin 6B Gene ID (NCBI): 3854 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P04259 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.