Proteintech, 85630-6-RR, RNF20 Recombinant monoclonal antibody

Size: 20ul / 100ul The RNF20 (85630-6-RR) by Proteintech is a Recombinant antibody targeting RNF20 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 85630-6-RR targets RNF20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, HeLa cells, Jurkat cells, MCF-7 cells, HEK-293 cells, NIH/3T3 cells. C2C12 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U-87 MG cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information RNF20, also named as E3 ubiquitin-protein ligase BRE1A, is a 975 amino acid protein, which contains 1 RING-type zinc finger and belongs to the BRE1 family. RNF20 is a component of the RNF20/40 complex and localizes in the nucleus. RNF20 is a component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B. RNF20 is required for transcriptional activation of Hox genes and recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. RNF20/40 complex monoubiquitinates and stabilizes Eg5. Loss of RNF20/40 results in spindle assembly defects, cell cycle arrest and apoptosis. Spindle assembly role of the RNF20/40 complex, and implicates the RNF20/40-Eg5 axis in breast carcinogenesis, supporting the pursuit of these proteins as potential targets for breast cancer therapeutic interventions. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag16066 Product name: Recombinant human RNF20 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 96-433 aa of BC110585 Sequence: YWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQDFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQHQVELIERDEVSLHKK Predict reactive species Full Name: ring finger protein 20 Calculated Molecular Weight: 975 aa, 114 kDa Observed Molecular Weight: 114 kDa GenBank Accession Number: BC110585 Gene Symbol: RNF20 Gene ID (NCBI): 56254 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q5VTR2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.