Proteintech, 85640-4-RR, SF3B1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SF3B1 (85640-4-RR) by Proteintech is a Recombinant antibody targeting SF3B1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 85640-4-RR targets SF3B1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, U2OS cells, Jurkat cells, K-562 cells, HeLa cells, NIH/3T3 cells, C6 cells Positive IF/ICC detected in: MCF-7 cells, NIH/3T3 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag26689 Product name: Recombinant human SF3B1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-126 aa of NM_012433 Sequence: MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTM Predict reactive species Full Name: splicing factor 3b, subunit 1, 155kDa Calculated Molecular Weight: 146 kDa Observed Molecular Weight: 146 kDa GenBank Accession Number: NM_012433 Gene Symbol: SF3B1 Gene ID (NCBI): 23451 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O75533 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.