Proteintech, 85664-4-RR, SII-TFIIS,TCEA1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SII-TFIIS,TCEA1 (85664-4-RR) by Proteintech is a Recombinant antibody targeting SII-TFIIS,TCEA1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 85664-4-RR targets SII-TFIIS,TCEA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, SGC-7901 cells, K-562 cells, Sp2/0 cells Positive IF/ICC detected in: A431 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag12199 Product name: Recombinant human SII-TFIIS,TCEA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC072460 Sequence: MEDEVVRFAKKMDKMVQKKNAAGALDLLKELKNIPMTLELLQSTRIGMSVNAIRKQSTDEEVTSLAKSLIKSWKKLLDGPSTEKDLDEKKKEPAITSQNSPEAREESTSSGNVSNRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYIAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADEPMTTFVVCNECGNRWKFC Predict reactive species Full Name: transcription elongation factor A (SII), 1 Calculated Molecular Weight: 301 aa, 34 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC072460 Gene Symbol: TCEA1 Gene ID (NCBI): 6917 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P23193 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.