Proteintech, 85703-1-RR, Phospho-ATG4B (Ser383) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-ATG4B (Ser383) (85703-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-ATG4B (Ser383) in WB, FC (Intra), ELISA applications with reactivity to human samples 85703-1-RR targets Phospho-ATG4B (Ser383) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells Positive FC (Intra) detected in: λ phosphatase treated K-562 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information ATG4B stimulates autophagy by promoting autophagosome formation through reversible modification of ATG8. In humans, microtubule-associated protein 1 light chain, LC3B, is the best-characterized ATG8 isoform, and ATG4B, but not three other ATG4 isoforms, displays a highly selective preference toward LC3B. MST4 phosphorylates ATG4B at serine residue 383, which stimulates ATG4B activity and increases autophagic flux. (PMID: 29232556, PMID: 36056541) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ATG4 autophagy related 4 homolog B (S. cerevisiae) Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 44-48 kDa GenBank Accession Number: BC000719 Gene Symbol: ATG4B Gene ID (NCBI): 23192 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y4P1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.