Proteintech, 85768-2-RR, INVS Recombinant monoclonal antibody

Size: 20ul / 100ul The INVS (85768-2-RR) by Proteintech is a Recombinant antibody targeting INVS in WB, IP, ELISA applications with reactivity to human samples 85768-2-RR targets INVS in WB, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, SH-SY5Y cells, K-562 cells Positive IP detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of the left-right axis. INVS may be involved in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0934 Product name: Recombinant human INVS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-101 aa of BC006370 Sequence: MNKSENLLFAGSSLASQVHAAAVNGDKGALQRLIVGNSALKDKEDQFGRTPLMYCVLADRLDCADALLKAGADVNKTDHSQRTALHLAAQKALRTISTGRI Predict reactive species Full Name: inversin Calculated Molecular Weight: 118 kDa, 100 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC006370 Gene Symbol: INVS Gene ID (NCBI): 27130 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y283 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.