Proteintech, 85782-2-RR, C4b Alpha Chain/C4b-B/C4d-B Recombinant monoclonal antibody

Size: 20ul / 100ul The C4b Alpha Chain/C4b-B/C4d-B (85782-2-RR) by Proteintech is a Recombinant antibody targeting C4b Alpha Chain/C4b-B/C4d-B in WB, IF/ICC, ELISA applications with reactivity to human samples 85782-2-RR targets C4b Alpha Chain/C4b-B/C4d-B in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human blood Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single-chain precursor, which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1116-1128aa of human C4b protein can recognize C4b precursor, C4b alpha chain, C4b-B, and C4d-B. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: complement component 4B (Chido blood group) Calculated Molecular Weight: 193 kDa Observed Molecular Weight: 95 kDa GenBank Accession Number: NM_000592 Gene Symbol: C4B Gene ID (NCBI): 721 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P0C0L5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.