Proteintech, 85945-1-RR, RDBP Recombinant monoclonal antibody

Size: 20ul / 100ul The RDBP (85945-1-RR) by Proteintech is a Recombinant antibody targeting RDBP in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 85945-1-RR targets RDBP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, Calu-3 cells, mouse brain tissue, rat brain tissue, HEK-293 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2500-1:7500 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1186 Product name: Recombinant human RDBP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-333 aa of BC011600 Sequence: MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF Predict reactive species Full Name: RD RNA binding protein Calculated Molecular Weight: 37 kDa, 43 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC011600 Gene Symbol: NELFE Gene ID (NCBI): 7936 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P18615 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.