Proteintech, 85963-1-RR, ENPEP Recombinant monoclonal antibody

Size: 20ul / 100ul The ENPEP (85963-1-RR) by Proteintech is a Recombinant antibody targeting ENPEP in WB, ELISA applications with reactivity to human, mouse, rat samples 85963-1-RR targets ENPEP in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue, human kidney tissue, rat lung tissue, rat kidney tissue, mouse lung tissue, = Recommended dilution Western Blot (WB): WB : 1:1000-1:2000 Background Information ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg2736 Product name: Recombinant Human ENPEP protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 41-957 aa of NM_001977.3 Sequence: RSCDSSGDGGPGTAPAPSHLPSSTASPSGPPAQDQDICPASEDESGQWKNFRLPDFVNPVHYDLHVKPLLEEDTYTGTVSISINLSAPTRYLWLHLRETRITRLPELKRPSGDQVQVRRCFEYKKQEYVVVEAEEELTPSSGDGLYLLTMEFAGWLNGSLVGFYRTTYTENGQVKSIVATDHEPTDARKSFPCFDEPNKKATYTISITHPKEYGALSNMPVAKEESVDDKWTRTTFEKSVPMSTYLVCFAVHQFDSVKRISNSGKPLTIYVQPEQKHTAEYAANITKSVFDYFEEYFAMNYSLPKLDKIAIPDFGTGAMENWGLITYRETNLLYDPKESASSNQQRVATVVAHELVHQWFGNIVTMDWWEDLWLNEGFASFFEFLGVNHAETDWQMRDQMLLEDVLPVQEDDSLMSSHPIIVTVTTPDEITSVFDGISYSKGSSILRMLEDWIKPENFQKGCQMYLEKYQFKNAKTSDFWAALEEASRLPVKEVMDTWTRQMGYPVLNVNGVKNITQKRFLLDPRANPSQPPSDLGYTWNIPVKWTEDNITSSVLFNRSEKEGITLNSSNPSGNAFLKINPDHIGFYRVNYEVATWDSIATALSLNHKTFSSADRASLIDDAFALARAQLLDYKVALNLTKYLKREENFLPWQRVISAVTYIISMFEDDKELYPMIEEYFQGQVKPIADSLGWNDAGDHVTKLLRSSVLGFACKMGDREALNNASSLFEQWLNGTVSLPVNLRLLVYRYGMQNSGNEISWNYTLEQYQKTSLAQEKEKLLYGLASVKNVTLLSRYLDLLKDTNLIKTQDVFTVIRYISYNSYGKNMAWNWIQLNWDYLVNRYTLNNRNLGRIVTIAEPFNTELQLWQMESFFAKYPQAGAGEKPREQVLETVKNNIEWLKQHRNTIREWFFNLLESG Predict reactive species Full Name: glutamyl aminopeptidase (aminopeptidase A) Calculated Molecular Weight: 109 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: NM_001977.3 Gene Symbol: ENPEP Gene ID (NCBI): 2028 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q07075 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.