Proteintech, 85994-1-RR, NPTXR Recombinant monoclonal antibody

Size: 20ul / 100ul The NPTXR (85994-1-RR) by Proteintech is a Recombinant antibody targeting NPTXR in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse samples 85994-1-RR targets NPTXR in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: IMR-32 cells, U-87 MG cells, mouse brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information NPTXR (Neuronal Pentraxin Receptor) is a type II transmembrane glycoprotein primarily expressed in the cerebellum and hippocampus. It mediates synaptic plasticity by clustering AMPA-type glutamate receptors at excitatory synapses and promoting synaptogenesis. NPTXR can be proteolytically cleaved to release a soluble form. Its dysregulation is implicated in Alzheimer's disease and small-cell lung cancer.(PMID: 9261167; 18367087; 19368810; 16115696) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg3109 Product name: Recombinant Human NPTXR protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 24-500 aa of NM_014293.3 Sequence: ASVPLAASPARALPGGADNASVASGAAASPGPQRSLSALHGAGGSAGPPALPGAPAASAHPLPPGPLFSRFLCTPLAAACPSGAQQGDAAGAAPGEREELLLLQSTAEQLRQTALQQEARIRADQDTIRELTGKLGRCESGLPRGLQGAGPRRDTMADGPWDSPALILELEDAVRALRDRIDRLEQELPARVNLSAAPAPVSAVPTGLHSKMDQLEGQLLAQVLALEKERVALSHSSRRQRQEVEKELDVLQGRVAELEHGSSAYSPPDAFKISIPIRNNYMYARVRKALPELYAFTACMWLRSRSSGTGQGTPFSYSVPGQANEIVLLEAGHEPMELLINDKVAQLPLSLKDNGWHHICIAWTTRDGLWSAYQDGELQGSGENLAAWHPIKPHGILILGQEQDTLGGRFDATQAFVGDIAQFNLWDHALTPAQVLGIANCTAPLLGNVLPWEDKLVEAFGGATKAAFDVCKGRAKA Predict reactive species Full Name: neuronal pentraxin receptor Calculated Molecular Weight: 53 kDa Observed Molecular Weight: 35-65 kDa GenBank Accession Number: NM_014293.3 Gene Symbol: NPTXR Gene ID (NCBI): 23467 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O95502 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.