Proteintech, 86038-1-RR, CCR3 Recombinant monoclonal antibody

Size: 20ul / 100ul The CCR3 (86038-1-RR) by Proteintech is a Recombinant antibody targeting CCR3 in WB, IF/ICC, ELISA applications with reactivity to human samples 86038-1-RR targets CCR3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A375 cells, THP-1 cells, Jurkat cells, HUVEC cells, U-87 MG cells Positive IF/ICC detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information CCR3 (C-C Motif Chemokine Receptor 3), also known as CD193, is a G protein-coupled receptor (GPCR) primarily involved in immune cell trafficking and inflammation regulation and highly expressed in eosinophils and basophils, with detectable levels in Th2 cells, airway epithelial cells, and subsets of monocyte-derived dendritic cells (PMID: 21077277). Ligand-induced CCR3 internalization is required for eosinophil shape change and actin polymerization but not for calcium mobilization or desensitization (PMID: 12532103). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: chemokine (C-C motif) receptor 3 Calculated Molecular Weight: 355 aa, 41 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC110297 Gene Symbol: CCR3 Gene ID (NCBI): 1232 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P51677 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.