Proteintech, 86057-1-RR, Peripherin Recombinant monoclonal antibody

Size: 20ul / 100ul The Peripherin (86057-1-RR) by Proteintech is a Recombinant antibody targeting Peripherin in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 86057-1-RR targets Peripherin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: IMR-32 cells, mouse colon tissue, rat colon tissue Positive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse colon tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)-P: IF-P : 1:500-1:2000 Background Information Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag11365 Product name: Recombinant human Peripherin protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 155-470 aa of BC032703 Sequence: LELLGRERDRVQVERDGLAEDLAALKQRLEEETRKREDAEHNLVLFRKDVDDATLSRLELERKIESLMDEIEFLKKLHEEELRDLQVSVESQQVQQVEVEATVKPELTAALRDIRAQYESIAAKNLQEAEEWYKSKYADLSDAANRNHEALRQAKQEMNESRRQIQSLTCEVDGLRGTNEALLRQLRELEEQFALEAGGYQAGAARLEEELRQLKEEMARHLREYQELLNVKMALDIEIATYRKLLEGEESRISVPVHSFASLNIKTTVPEVEPPQDSHSRKTVLIKTIETRNGEVVTESQKEQRSELDKSSAHSY Predict reactive species Full Name: peripherin Calculated Molecular Weight: 470 aa, 54 kDa Observed Molecular Weight: 54-60 kDa GenBank Accession Number: BC032703 Gene Symbol: Peripherin Gene ID (NCBI): 5630 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P41219 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.