Proteintech, 86238-2-RR, Pan-Keratin (Type I) Recombinant monoclonal antibody

Size: 20ul / 100ul The Pan-Keratin (Type I) (86238-2-RR) by Proteintech is a Recombinant antibody targeting Pan-Keratin (Type I) in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 86238-2-RR targets Pan-Keratin (Type I) in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HaCaT cells, A431 cells, HT-29 cells, mouse skin tissue, rat skin tissue, rat colon tissue Positive IHC detected in: human prostate cancer tissue, Human Kidney(renal cell) Cancer, human tonsil tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human cervical cancer tissue, human colon cancer tissue, human lung cancer tissue, mouse skin tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:250-1:1000 Background Information Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type I keratins (or Type I cytokeratins) are acidic, low molecular weight (40-64 kDa) proteins that form Type I intermediate filaments (IFs) in the cytoplasmic cytoskeleton of all mammalian epithelial cells. They are encoded on chromosome 17q and include keratins K9-K10, K12-K28, and K31-K40. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Pan-Keratin (Type I) Observed Molecular Weight: 45-65 kDa Gene ID (NCBI): Pan-Keratin (Type I) Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15323 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.