Proteintech, 86277-2-RR, Cytokeratin 39 Recombinant monoclonal antibody

Size: 20ul / 100ul The Cytokeratin 39 (86277-2-RR) by Proteintech is a Recombinant antibody targeting Cytokeratin 39 in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 86277-2-RR targets Cytokeratin 39 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: T-47D cells, mouse skin tissue, rat skin tissue, HeLa cells, MCF-7 cells, A431 cells, HaCaT cells Positive IHC detected in: mouse esophagus tissue, human skin tissue, mouse skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse skin tissue Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Cytokeratin 39 (CK-39) is a member of the cytokeratin family, a group of intermediate filament proteins primarily expressed in epithelial cells. Cytokeratins are classified into Type I (acidic) and Type II (basic) subfamilies, which pair to form heteropolymers essential for maintaining cellular structural integrity, mechanical stability, and stress resistance. CK-39 is predominantly expressed in stratified and glandular epithelia, such as those found in the prostate, bladder, and respiratory tract. Its expression pattern may vary depending on tissue differentiation states. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: keratin 39 Observed Molecular Weight: 45 kDa GenBank Accession Number: BC157887 Gene Symbol: KRT39 Gene ID (NCBI): 390792 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6A163 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.