Proteintech, 86411-1-RR, BMP-9 Recombinant monoclonal antibody

Size: 20ul / 100ul The BMP-9 (86411-1-RR) by Proteintech is a Recombinant antibody targeting BMP-9 in WB, ELISA applications with reactivity to human, mouse, rat, pig samples 86411-1-RR targets BMP-9 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: mouse liver tissue, rat liver tissue, pig liver tissue, mouse brain tissue, HepG2 cells, L02 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Bone morphogenetic protein-9 (BMP9, also termed growth-differentiation factor-2, GDF-2) is a secreted signaling molecule that belongs to the transforming growth factor-β (TGF-β) super-family. First identified in fetal mouse liver cDNA libraries, BMP9 is predominantly produced by hepatic stellate cells and circulates at relatively high concentrations in adult plasma. BMP9 is synthesized as a precursor protein (pre-proBMP9) composed of 429 amino acids (aa) that includes a 22 aa signal peptide, a 297 aa prodomain (33 kDa) and a 110 aa mature protein (12.5 kDa). It is then cleaved by serine endoproteases, leading to a short dimeric mature form of 25 kDa, and the prodomain which can remain noncovalently associated with the mature short form, giving a size of about 100 kDa (PMID:21710321). Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg2482 Product name: recombinant mouse Gdf2 protein Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 23-428 aa of NM_019506.4 Sequence: KPLQNWEQASPGENAHSSLGLSGAGEEGVFDLQMFLENMKVDFLRSLNLSGIPSQDKTRAEPPQYMIDLYNRYTTDKSSTPASNIVRSFSVEDAISTAATEDFPFQKHILIFNISIPRHEQITRAELRLYVSCQNDVDSTHGLEGSMVVYDVLEDSETWDQATGTKTFLVSQDIRDEGWETLEVSSAVKRWVRADSTTNKNKLEVTVQSHRESCDTLDISVPPGSKNLPFFVVFSNDRSNGTKETRLELKEMIGHEQETMLVKTAKNAYQVAGESQEEEGLDGYTAVGPLLARRKRSTGASSHCQKTSLRVNFEDIGWDSWIIAPKEYDAYECKGGCFFPLADDVTPTKHAIVQTLVHLKFPTKVGKACCVPTKLSPISILYKDDMGVPTLKYHYEGMSVAECGCR Predict reactive species Full Name: growth differentiation factor 2 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: NM_019506.4 Gene Symbol: BMP9 Gene ID (NCBI): 12165 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9WV56 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.