Proteintech, 86435-1-RR, GMDS Recombinant monoclonal antibody

Size: 20ul / 100ul The GMDS (86435-1-RR) by Proteintech is a Recombinant antibody targeting GMDS in WB, ELISA applications with reactivity to human, mouse, rat samples 86435-1-RR targets GMDS in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-29 cells, HepG2 cells, HeLa cells, HEK-293 cells, A431 cells, mouse pancreas tissue, rat pancreas tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information GDP-D-mannose-4,6-dehydratase (GMDS) is the key enzyme in the 'de novo' pathway of GDP-L-fucose biosynthesis(PMID:10462046). The enzyme is also named as GMD and belongs to the GDP-mannose 4,6-dehydratase family. The gene encodes a 42 kDa protein that catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7319 Product name: Recombinant human GMDS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-372 aa of BC000117 Sequence: MAHAPARCPSARGSGDGEMGKPRNVALITGITGQDGSYLAEFLLEKGYEVHGIVRRSSSFNTGRIEHLYKNPQAHIEGNMKLHYGDLTDSTCLVKIINEVKPTEIYNLGAQSHVKISFDLAEYTADVDGVGTLRLLDAVKTCGLINSVKFYQASTSELYGKVQEIPQKETTPFYPRSPYGAAKLYAYWIVVNFREAYNLFAVNGILFNHESPRRGANFVTRKISRSVAKIYLGQLECFSLGNLDAKRDWGHAKDYVEAMWLMLQNDEPEDFVIATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKETGKVHVTVDLKYYRPTEVDFLQGDCTKAKQKLNWKPRVAFDELVREMVHADVELMRTNPNA Predict reactive species Full Name: GDP-mannose 4,6-dehydratase Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC000117 Gene Symbol: GMDS Gene ID (NCBI): 2762 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O60547 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.