Proteintech, 86457-4-RR, Cytokeratin 8 Recombinant monoclonal antibody

Size: 20ul / 100ul The Cytokeratin 8 (86457-4-RR) by Proteintech is a Recombinant antibody targeting Cytokeratin 8 in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 86457-4-RR targets Cytokeratin 8 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A431 cell, HaCaT cells, MCF-7 cells, C6 cells, mouse skin tissue, rat skin tissue Positive IHC detected in: human tonsil tissue, human breast cancer tissue, Human Kidney(renal cell) Cancer, mouse liver tissue, rat liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human breast cancer tissue Positive IF/ICC detected in: A431 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information Cytokeratin 8 (CK8) is a protein that belongs to the cytokeratin family, which is a group of structural proteins found in the intracytoplasmic cytoskeleton of epithelial cells. CK8 forms intermediate filaments within the cytoplasm of simple epithelial cells, typically as a dimer with cytokeratin 18 (CK18). These filaments provide a stabilizing framework that helps determine cell shape and enables cells to cope with mechanical stress. CK8/CK18 filaments also serve as a mesh of "paths" for signaling molecules, metabolites, and pathogens to travel within the cell in an organized manner. CK8 is expressed in various epithelial tissues and is particularly associated with glandular and simple epithelia. Its expression is also a significant marker in several types of cancer, including lung and esophageal cancer, where it can indicate tumor progression and poor prognosis. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: keratin 8 Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC000654 Gene Symbol: Cytokeratin 8 Gene ID (NCBI): 3856 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P05787 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.