Proteintech, 86513-3-RR, CPSF2 Recombinant monoclonal antibody

Size: 20ul / 100ul The CPSF2 (86513-3-RR) by Proteintech is a Recombinant antibody targeting CPSF2 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86513-3-RR targets CPSF2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, SH-SY5Y cells, Ramos cells, RAW 264.7 cells, C6 cells, mouse brain tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Cleavage and Polyadenylation Specificity Factor Subunit 2 (CPSF2) is a critical component of the mRNA 3′ end processing machinery, playing a pivotal role in the cleavage and polyadenylation of pre-mRNA. This process is essential for the stability, nuclear export, and translation efficiency of mRNA, making CPSF2 a key player in gene expression regulation. Recent studies have highlighted the significance of CPSF2 in various diseases, particularly cancer. It's reported that in papillary thyroid carcinoma (PTC), negative expression of CPSF2 has been associated with poorer clinical outcomes, including higher rates of cervical lymph node metastasis, distant metastasis, and reduced recurrence-free survival. (PMID:26148673) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag11950 Product name: Recombinant human CPSF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 433-782 aa of BC070095 Sequence: SAHKTKHDLMMKGEGSRKGSFFKQAKKSYPMFPAPEERIKWDEYGEIIKPEDFLVPELQATEEEKSKLESGLTNGDEPMDQDLSDVPTKCISTTESIEIKARVTYIDYEGRSDGDSIKKIINQMKPRQLIIVHGPPEASQDLAECCRAFGGKDIKVYMPKLHETVDATSETHIYQVRLKDSLVSSLQFCKAKDAELAWIDGVLDMRVSKVDTGVILEEGELRDDGEDSEMQVEAPSDSSVIAQQKAMKSLFGDDEKETGEESEIIPTLEPLPPHEVPGHQSVFMNEPRLSDFKQVLLREGIQAEFVGGVLVCNNQVAVRRTETGRIGLEGCLCQDFYRIRDLLYEQYAIV Predict reactive species Full Name: cleavage and polyadenylation specific factor 2, 100kDa Calculated Molecular Weight: 782 aa, 88 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC070095 Gene Symbol: CPSF2 Gene ID (NCBI): 53981 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9P2I0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.