Proteintech, 86680-2-RR, POLB Recombinant monoclonal antibody

Size: 20ul / 100ul The POLB (86680-2-RR) by Proteintech is a Recombinant antibody targeting POLB in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86680-2-RR targets POLB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: C6 cells, mouse spleen tissue, rat spleen tissue, mouse brain tissue, rat brain tissue Positive IF/ICC detected in: COLO 320 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information DNA polymerase β (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation (PMID: 38731327). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag12496 Product name: Recombinant human POLB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-335 aa of BC100288 Sequence: MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE Predict reactive species Full Name: polymerase (DNA directed), beta Calculated Molecular Weight: 335 aa, 38 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC100288 Gene Symbol: DNA Polymerase Beta Gene ID (NCBI): 5423 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P06746 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.